Monthly Archives: August 2018

Genetic Transformation and Tool Development

Overview:  The ability to genetically modify microorganisms is foundational to performing rational strain engineering, but most organisms are not transformable when first isolated. We are able to develop initial transformation methodologies and protocols for new organisms or improve upon existing ones. We can further develop high throughput tools for more rapid strain modification in these […]

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Fungal Genomics, Synthetic Biology and Bioprocess Development

Overview:  The ABF houses the filamentous fungal and non-model yeast synthetic biology capability unique within the government laboratories. This effort also comprises integrated fungal genetic tool and medium scale (15-120L) bioprocess development capability. Samples for Testing and Learning by a systems biology approach are generated from large shake flask experimental capabilities or through one of […]

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DIVA DNA Sequence Validation

  Overview:  Comprises sequence validation of plasmid and/or amplicon DNA using the Illumina MiSeq platform. Customized ABF workflow minimizes library preparation steps and leverages laboratory automation to enable scale/reduce reagent costs, provides full plasmid coverage, and is integrated with the ICE sequence/strain repository platform. This validates DNA sequences (ensures that they actually are what they […]

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DIVA DNA Construction

Overview:  This capability relates to physical construction of sequence-validated plasmids. The workflow leverages laboratory automation to enable scale/reduce reagent costs, sequence validation provides full plasmid coverage, and is integrated with the DIVA/DeviceEditor/BOOST/j5 design, BLiSS biosecurity screening, and ICE sequence/strain repository platforms. This enables ABF researchers to focus their efforts where they are competitively advantaged (upstream […]

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DIVA bioCAD

Overview: DIVA is a software platform that brings together a tool-chain that enables the design of (combinatorial) DNA libraries and automates the generation of protocols to construct them. It brings together a tool-chain of DNA component repositories and visualization tools (e.g., ICE/VectorEditor), visual design tools (e.g., DeviceEditor), DNA construction optimization tools (e.g., BOOST, j5), and […]

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Industry Listening Day – March 15, 2016

On March 15, 2016, the national labs held an Industry Listening Day in Berkeley, CA. The purpose of the Listening Day was to gather input from key industry stakeholders on the R&D barriers to building a robust, accessible biomanufacturing platform. The labs also asked industry attendees to provide input on mechanisms for working with the […]

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SynBio Foundry Vision Review – June 14, 2016

On June 14, 2016, the national labs will be hosting a review of the SynBio Foundry vision in Washington, DC. The SynBio Foundry, as proposed, is the manifestation of the Agile Biomanufacturing visioning efforts. During this review, key industry and federal government stakeholders will be invited  to provide feedback on the proposed national lab consortium, […]

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