DIVA DNA Sequence Validation

Verify new DNA and microbial strains

Verifying source DNA material before new DNA construction begins — and confirming that the sequence of a newly fabricated DNA construct is correct before its transformation into a host organism — can save substantial downstream time and resources spent working with flawed sequences.

Our DIVA DNA sequence validation service provides full NGS (no DNA oligos required) plasmid/amplicon sequence coverage with a turn around time of about 1.5 weeks, with up to 1536 samples per sequencing run.

An overrview of the DIVA DNA Sequence Validation workflow. A chart flowing from left to right shows the process as DNA input, library preparation, sequencing, and analysis.
An overview of the DIVA DNA Sequence Validation workflow

This capability is primarily intended to:

  • validate that a DNA plasmid has been constructed as desired
  • validate that DNA has been correctly integrated into a genomic locus (via sequencing and amplicon of the target locus)
  • otherwise verify the sequence of a plasmid or an amplicon

This service can be applied as part of a workflow to generate sequencing reads for counting statistics as part of a gene variant screening/selection process. It can also help detect enrichments or depletions of specific gene variants in the course of a screening or selection process.

While the availability of reference sequences (to be validated) is preferred, it is also possible to do de novo DNA sequence assembly when the reference sequence is unknown. This service is not intended to be used for microbial genome resequencing.

  • Lawrence Berkeley National Laboratory
  • Pacific Northwest National Laboratory
  • Sandia National Laboratories