FRET Biosensors

Enabling high-throughput screening and analysis for test and learn efforts

We can design and optimize FRET biosensors for a variety of target molecules using an in-house library of fluorescent protein scaffolds.

FRET-based biosensors couple ligand-dependent protein conformational changes to a ratiometric fluorescent signal response. Such biosensors can be used intra- or extracellularly to provide rapid, real-time responses to ligands or metabolites of interest.  

Unlike typical reporter systems, signals from FRET biosensors are reversible, permitting detection of anabolic and catabolic (conversion) processes and variations in metabolite fluxes.  

Sensors for a variety of metabolites are available and new sensors directed towards new metabolites could be readily developed through collaboration.


  • Argonne National Laboratory