Droplet-based microfluidics is used to survey cell-based or cell-free expression libraries of the 1×104 – 1×106 size in picoliter aqueous droplets to identify rare events. The system can identify enzymes with promiscuous activities and can be used for protein engineering. The droplet-based microfluidics complements FACS-based methods, and supports high-throughput protein engineering. A fluorescent-based reporter assay is necessary to perform the survey. This capability is available for collaborations with the ABF.
Argonne National Laboratory