Multiscale Bioreactor Cultivation

Multiscale Bioreactor Cultivation


The ABF has multiple scales of integrated bioreactor cultivation equipment ranging from the microscale BioLector Pro systems (2 mL cultivation volumes), more than 30 0.5-L fully controlled bioreactors, multiple 2 L, 5 L, and 10 L scales, up to 100, 1,000, and 9,000 L reactors in the Integrated Biorefinery Refinery Facility that opérate on an as-needed basis.  These include aerobic and anaerobic processes and are routinely used for multi-omics investigations of non-model strains on feedstocks ranging from clean sugars to biomass hydrolysates to non-standard substrates (e.g., waste gas, lignin-derived liquor, etc.). In situ product recovery is also available for bench scale units. This capability benefits the ABF mission by enabling rapid strain evaluation and multiscale, multi-omics investigations of non-model strains.

References and Additional Information: 

Eric M. Karp‡, Todd R. Eaton‡, Violeta Sànchez i Nogué‡, Vassili Vorotnikov‡, Mary J. Biddy, Eric C.D. Tan, David G. Brandner, Robin M. Cywar, Rongming Liu, Lorenz P. Manker, William E. Michener, Michelle Gilhespy, Zinovia Skoufa, Michael J. Watson, O. Stanley Fruchey, Derek R. Vardon, Ryan T. Gill, Adam J. Bratis, Gregg T. Beckham, “Renewable acrylonitrile production”, Science. (2017) 358, 1307-1310.

Michael T. Guarnieri, Yat-Chen C. Chou, Davinia Salvachua, Ali Mohagheghi, Peter St. John, Yannick J. Bomble, Gregg T. Beckham, “Metabolic engineering of Actinobacillus succinogenes provides insights into succinic acid biosynthesis”, Appl. Env. Microbiol. (2017) 83(17), e00996-17.

Robert Nelson, Darren Peterson, Eric M. Karp, Gregg T. Beckham, Davinia Salvachúa, “Mixed carboxylic acid production by Megasphaera elsdenii from glucose and lignocellulosic hydrolysate”, in press, Fermentation. (2017) 3(1), 10.


Lawrence Berkeley National Laboratory

National Renewable Energy Laboratory