Kristin E. Burnum-Johnson is a Senior Scientist and Team Lead at the Environmental Molecular Sciences Laboratory, a Department of Energy user facility at Pacific Northwest National Laboratory (PNNL). Kristin earned her Ph.D. in Biochemistry from Vanderbilt University with Richard M. Caprioli in 2008, and then completed a postdoctoral fellowship at PNNL with Richard D. Smith. At PNNL, her research is dedicated to characterizing the molecular landscape of heterogeneous samples using novel mass spectrometry approaches to address specific biological, medical, and environmental research questions. These approaches involve multi-dimensional liquid chromatography and ion mobility separations in conjunction with mass spectrometry to measure proteins, lipids, and metabolites. In the Agile BioFoundry, Kristin directs Test Tasks to support DBTL efforts.
Projects in the Agile BioFoundry
- Proteomics, metabolomics, and lipidomics
Yuqian Gao , Thomas L. Fillmore, Nathalie Munoz, Gayle J. Bentley, Christopher W. Johnson, Joonhoon Kim, Jamie A. Meadows, Jeremy D. Zucker, Meagan C. Burnet, Anna K. Lipton, Aivett Bilbao, Daniel J. Orton, Young-Mo Kim, Ronald J. Moore, Errol W. Robinson, Scott E. Baker, Bobbie-Jo M. Webb-Robertson, Adam M. Guss, John M. Gladden, Gregg T. Beckham, Jon K. Magnuson, Kristin E. Burnum-Johnson. “High-Throughput Large-Scale Targeted Proteomics Assays for Quantifying Pathway Proteins in Pseudomonas putida KT2440”. Frontiers in Bioengineering and Biotechnology. 2020;8. https://doi.org/10.3389/fbioe.2020.603488
Paul D. Piehowski, Ying Zhu, Lisa M. Bramer, Kelly G. Stratton, Rui Zhao, Daniel J. Orton, Ronald J. Moore, Jia Yuan, Hugh D. Mitchell, Yuqian Gao, Bobbie-Jo M. Webb-Robertson, Sudhansu K. Dey, Ryan T. Kelly, Kristin E. Burnum-Johnson. “Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution”. Nature Communications. 2020;11:8. https://doi.org/10.1038/s41467-019-13858-z
Kristin E. Burnum-Johnson, Xueyun Zheng, James N. Dodds, Jeremy Ash, Denis Fourches, Carrie D. Nicora, Jason P. Wendler, Thomas O. Metz, Katrina M. Waters, Janet K. Jansson, Richard D. Smith, Erin S. Baker. “Ion mobility spectrometry and the omics: Distinguishing isomers, molecular classes and contaminant ions in complex samples”. TrAC Trends in Analytical Chemistry. 2019;116:292-9. https://doi.org/10.1016/j.trac.2019.04.022
Kristin E. Burnum-Johnson, Jennifer E. Kyle, Amie J. Eisfeld, Cameron P. Casey, Kelly G. Stratton, Juan F. Gonzalez, Fabien Habyarimana, Nicholas M. Negretti, Amy C. Sims, Sadhana Chauhan, Larissa B. Thackray, Peter J. Halfmann, Kevin B. Walters, Young-Mo Kim, Erika M. Zink, Carrie D. Nicora, Karl K. Weitz, Bobbie-Jo M. Webb-Robertson, Ernesto S. Nakayasu, Brian Ahmer, Michael E. Konkel, Vladimir Motin, Ralph S. Baric, Michael S. Diamond, Yoshihiro Kawaoka, Katrina M. Waters, Richard D. Smith, Thomas O. Metz. “MPLEx: a method for simultaneous pathogen inactivation and extraction of samples for multi-omics profiling”. Analyst. 2017;142:442-8. https://doi.org/10.1039/C6AN02486F
Kristin E. Burnum-Johnson, Song Nie, Cameron P. Casey, Matthew E. Monroe, Daniel J. Orton, Yehia M. Ibrahim, Marina A. Gritsenko, Therese R. W. Clauss, Anil K. Shukla, Ronald J. Moore, Samuel O. Purvine, Tujin Shi, Weijun Qian, Tao Liu, Erin S. Baker, Richard D. Smith. “Simultaneous Proteomic Discovery and Targeted Monitoring using Liquid Chromatography, Ion Mobility Spectrometry, and Mass Spectrometry”. Molecular & Cellular Proteomics. 2016;15:3694-705. https://doi.org/10.1074/mcp.M116.061143