Pathway Development and Evaluation
Develop metabolic engineering strategies for optimized production of target molecules
Our pathway development and evaluation capabilities involve designing metabolic engineering and bioprocessing strategies for the production of exemplar target molecules associated with high flux metabolic intermediates called beachheads, which microbial metabolism can convert into several possible targets.
By focusing on exemplar targets, we can rapidly develop strategies for the production of related bioproducts. Rather than starting from scratch, our collaborators can leverage existing strategies, processes, and techno-economic and life cycle assessments developed for an exemplar target associated with the same beachhead.
View a full-size PDF of the metabolic map.
USE CASE
A comparison of the minimum selling prices of adipic acid (AA) and muconic acid (MA) from previous publications and those determined in a 2024 Agile BioFoundry study.
The Agile BioFoundry has made significant progress in developing strains for high yield production of muconic acid.
We have lowered the minimum selling price for adipic acid derived from catalytic hydrogenation of muconic acid to as low as $2.60 per kg, approaching cost parity.
Our process reduces greenhouse gas emissions by up to 80% compared with fossil-derived adipic acid.
Publications
- Bioprocess development and scale-up for cis,cis-muconic acid production from glucose and xylose by Pseudomonas putida
- Innovative Chemicals and Materials from Bacterial Aromatic Catabolic Pathways
- Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering
- Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440