Droplet Adaptive Laboratory Evolution

A powerful technique to improve bioproduction

Droplet Adaptive Laboratory Evolution (dALE) can be used to isolate desired phenotypes, such as higher productivity and feedstock and/or product tolerance.

Individual cells are encapsulated and cultivated in 1-30 pL droplets, enabling the generation of more than 10 million independent cultures. The cells are recovered from the final round and hundreds of isolates are characterized via an automated high throughput method for growth and productivity.

A combination of traditional strain engineering and adaptive laboratory evolution is a powerful technique to improve bioproduction.

  • Argonne National Laboratory