John Gladden

Sandia National Laboratories

Research Focus

John Gladden is a Principal Member of the Technical Staff at NTESS. He received his PhD in Molecular and Cell Biology at UC Berkeley in 2005 and has almost 20 years of experience in the field. Since 2008 John has focused on developing technologies for the conversion of lignocellulose into biofuels and bioproducts. He has lead interdisciplinary teams of biologists, computer scientists, and chemical engineers, both at Sandia and in partnership with other National Labs, to advance the state of the art in areas of biomass pretreatment and enzymatic depolymerization, sugar and lignin catabolism, and metabolic engineering of non-model organisms to convert depolymerize lignocellulose into biofuels and bioproducts. In the Agile Biofoundry, John co-leads the DBTL Task and is lead of the Rhodosporidium toruloides DBTL efforts.

Projects in the Agile BioFoundry

  • NTESS Principal Investigator for the Agile BioFoundry
  • Co-Lead of the DBTL Task
  • Lead of the Rhodosporidium toruloides DBTL efforts
  • Supervisor of the Experimental Data Depot NTESS development team

Featured Publications

Junko Yaegashi, James Kirby, Masakazu Ito, Jian Sun, Tanmoy Dutta, Mona Mirsiaghi, Eric R. Sundstrom, Alberto Rodriguez, Edward Baidoo, Deepti Tanjore, Todd Pray, Kenneth Sale, Seema Singh, Jay D. Keasling, Blake A. Simmons, Steven W. Singer, Jon K. Magnuson, Adam P. Arkin, Jeffrey M. Skerker and John M. Gladden, Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts, Biotechnology for Biofuels, 2017, 10:241,

Sebastian Kolinko, Yu-Wei Wu, Firehiwot Tachea,Evelyn Denzel, Jennifer Hiras, Raphael Gabriel, Nora Bäcker, Leanne Jade Chan, Stephanie A. Eichorst, Dario Frey, Qiushi Chen, Parastoo Azadi, Paul D. Adams, Todd R. Pray, Deepti Tanjore, Christopher J. Petzold, John M. Gladden, Blake A. Simmons, Steven W. Singer, A Bacterial Pioneer Produces Cellulase Complexes that Persist through Community Succession, Nature Microbiology, November 2017,

Michael Liszka, Aram Kang, Suryanarayana Konda, Kim Tran, John Gladden, Seema Singh, Jay D Keasling, Corinne D. Scown, Taek Soon Lee, Blake Simmons, Kenneth L Sale, Switchable Ionic Liquids Based on Di-Carboxylic Acids for One-Pot Conversion of Biomass to an Advanced Biofuel, Green Chemistry, published online April 2016, DOI: 10.1039/C6GC00657D.

Wu W, Tran W, Taatjes CA, Alonso-Gutierrez J, Lee TS, Gladden JM (2016) Rapid Discovery and Functional Characterization of Terpene Synthases from Four Endophytic Xylariaceae. PLoS ONE 11(2): e0146983. doi:10.1371/journal.pone.0146983.

John M Gladden, Joshua I Park, Jessica Bergmann, Vimalier Reyes-Ortiz, Patrik D’haeseleer, Betania F Quirino, Kenneth L Sale, Blake A Simmons and Steven W Singer, Discovery and Characterization of Ionic Liquid-Tolerant Thermophilic Cellulases from a Swithchrass-Adapted Microbial Community, Biotechnology for Biofuels, 2014 7:15

Joshua I. Park, Eric J. Steen, Helcio Burd, Sophia S. Evans, Alyssa M. Redding-Johnson, Tanveer Batth, Peter I. Benke, Patrik D’haeseleer, Ning Sun, Kenneth L. Sale, Jay D. Keasling, Taek Soon Lee, Christopher J. Petzold, Aindrila Mukhopadhyay, Terry C. Hazen, Steven W. Singer, Blake A. Simmons, and John Gladden. A Thermophilic Ionic liquid-tolerant Cellulase Cocktail for the Production of Cellulosic Biofuels, PLOS ONE, 2012, 7(5), e37010