DIVA DNA Sequence Construction and Validation

Physically fabricate and verify DNA constructs of interest

DIVA DNA Construction

Leverage our team’s domain expertise, laboratory automation, methods and workflows to physically fabricate and sequence validate DNA constructs of interest.

Our collaborators can submit DNA designs through DIVA bioCAD software to be constructed and sequence validated via the DIVA DNA Sequence Validation service, following a technical feasibility review and biosecurity screening. This may include sourcing synthetic DNA from commercial vendors.

Adjustments may be needed for a design before it can be constructed. Turnaround time and service capacity may vary depending on the complexity and size of a design, but 6 weeks turn around —including routine synthetic DNA fragment/oligo synthesis and sequence validation — is representative for a typical plasmid.

The Agile BioFoundry offers DNA construction at scales and efficiencies that might not otherwise be accessible. From an opportunity cost perspective, this capability liberates our collaborators to offload DNA construction tasks and dedicate time to those research activities they specialize in and are competitively advantaged.

DIVA DNA Sequence Validation 

Verifying source DNA material before new DNA construction begins — and confirming that the sequence of a newly fabricated DNA construct is correct before its transformation into a host organism — can save substantial downstream time and resources spent working with flawed sequences.

Our DIVA DNA sequence validation service provides full NGS (no DNA oligos required) plasmid/amplicon sequence coverage with a turn around time of about 1.5 weeks, with up to 1536 samples per sequencing run.

An overview of the DIVA DNA Sequence Validation workflow

This capability is primarily intended to:

  • validate that a DNA plasmid has been constructed as desired
  • validate that DNA has been correctly integrated into a genomic locus (via sequencing and amplicon of the target locus)
  • otherwise verify the sequence of a plasmid or an amplicon

This service can be applied as part of a workflow to generate sequencing reads for counting statistics as part of a gene variant screening/selection process. It can also help detect enrichments or depletions of specific gene variants in the course of a screening or selection process.

While the availability of reference sequences (to be validated) is preferred, it is also possible to do de novo DNA sequence assembly when the reference sequence is unknown. This service is not intended to be used for microbial genome resequencing.