Mechanistic Models of Metabolic Flux

Two-scale 13C Metabolic Flux Analysis (TS-13C MFA) measures intracellular metabolic fluxes by using data obtained from 13C substrate labeling experiments and genome-scale models. Metabolic fluxes map how carbon and electrons flow through metabolism to enable cell function and provide the best available insights to guide metabolic engineering. This is the only 13C labeling technique that has been used to produce flux measurements for genome-scale models and improve bioproduct yields. TS-13C MFA provides a mechanistic modeling approach that is proven to guide metabolic engineering efforts to improve bioproducts yields. TS-13C MFA is an accurate but slow capability that may take several months to produce results. Currently we can process 4-5 strains per semester. Code is available as part of the open source jQMM library.

  • Lawrence Berkeley National Laboratory
  • National Renewable Energy Laboratory
  • Pacific Northwest National Laboratory